Abstract
Study Objective
To evaluate the effect of fibroblast growth factor (FGF) on epithelialization of neovagina
in patients with Mayer-Rokitansky-Küster-Hauser syndrome who underwent vaginoplasty.
Design
Observational study.
Setting
University hospital.
Participants
Seven patients with Mayer-Rokitansky-Küster-Hauser syndrome.
Interventions
Cytological examination was done on vaginal smear samples from the site of completed
epithelialization, and tissue was collected from the epithelialized part for histological
evaluation. Immunostaining for estrogen receptor α, and keratin 13 and 14, and reverse
transcription polymerase chain reaction (RT-PCR) analysis of the FGF receptor (FGFR)
1-4 were performed in samples from case 2 three times (ie, during the surgery, during
the period of vaginal creation, and at 3 months and 6 months after the surgery).
Main Outcome Measures
The primary outcome was the FGF effects on the epithelialization speed and FGFR expression
in the neovagina. The second was the role of FGF in the mechanism of vaginal epithelial
cell proliferation.
Results
The histological structure of the neovagina was consistent with that of normal vagina.
RT-PCR analysis revealed that FGFR was expressed in the control vaginas and neovaginas.
Among the FGFR subtypes, FGFR-4 was overexpressed during the process of epithelialization
and its level decreased after completion of creation of the new vagina.
Conclusion
The epithelium of the neovagina was morphologically similar to that of normal vagina.
It is suggested that FGF plays the role as a growth factor.
Key Words
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Article info
Publication history
Published online: December 11, 2015
Footnotes
The authors indicate no conflicts of interest.
Identification
Copyright
© 2015 North American Society for Pediatric and Adolescent Gynecology. Published by Elsevier Inc.
